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Junior Research Fellow

 Bengaluru, Karnataka

Project

Multi-omic approaches and mass spectrometry based tissue metabolite imaging towards understanding metabolites localisation and metabolic pathways in specific cell/tissues of key medicinal plant, Dysoxylum binectariferum.

Profile

Advanced multi-omics technologies provide revolutionary strategies to study complex biological processes, e.g. to systematically decipher biosynthetic pathways to mechanistically challenging, structurally complex, widely used plant medicinals that cannot be economically synthesized. A multi-omics strategy is proposed to deduce biosynthetic pathway to rohitukine, a chromone alkaloid, widely used in cancer treatment. Distinct cell types involved in plantawill be identified using mass spectrometry based metabolite imaging in situand laser microscope dissection. Knowledge gained can be applied to developing synthetic biology approaches in order to obtain new sources of these compounds in other host systems.

Duration

One year contract, extendable up to next two years based on performance.

Money

As per SERB, DST norms

Experience

  • Molecular and biochemical techniques
  • Isolation, purification and structural characterization of secondary metabolitesExperience in Chromatographic techniques like HPLC, GCMS and LCMS
  • Experience in RNA isolation, Synthesis of cDNA. PCR, RT-PCR, qPCR, cloning and transformation.
  • Bioinformatics: transcriptome analysis

To Apply

Send out the detailed CV on or before the last date – 29.04.2019. All selected candidates will be informed. No TA/DA will be provided. Interested candidates to send their cv to mohan.kumar@tdu.edu.in

Posted on 02 Apr 2019.

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