We have planned a series of 7-Days workshops with practical hands-on training on the basic cell culture techniques; media preparation; genomic DNA
isolation, estimation and agarose gel electrophoresis; RNA
extraction, cDNA preparation, PCR
and agarose gel electrophoresis; protein extraction, estimation, SDS
and coomassie staining of proteins; freezing and thawing of cells; and MTT
Assay for proliferation/cytotoxicity. This is a basic workshop intended for beginners to teach and train them in animal cell culture and molecular techniques using the human/cancer cell lines for various experiments. It will be supported by some theoretical classes on basic cell culture techniques, media preparation, gene/protein expression &
interaction networks in hematopoietic stem cells (HSCs) and cancer cells.
Organised by: International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), Pune, India