Western blotting is a gold standard molecular biological technique, used for validating the presence of specific protein in the sample. Similarly, qPCR is a powerful sensitive technique used to quantify specific gene expressions. Western blotting and qPCR has a broad range of applications, including diagnostics, gene expression analysis, genotyping, and so on. In this context, this course will cover protein lysate preparation from tissues and cells, SDS-PAGE (Bio-Rad electrophoresis system), protein transfer (Bio-Rad transfer unit), western blotting (Bio-Rad ChemiDoc XRS+ System), qPCR primer designing, qPCR setup (Bio-Rad CFX Opus 96) and data analysis. This workshop will provide hands-on experience to learn the molecular biology techniques, especially SDS-PAGE, Western blotting and qPCR. Furthermore, this platform will help participants design qPCR / antibody-based diagnostic kits.