Prof. Paul D N Hebert is the Father of DNA Barcoding research and he proposed the above concept. The technology of DNA Barcoding is of very recent origin which facilitates species identification through the use of short, standardized gene region-mitochondrial Cytochrome C Oxidase Subunit I (COI). Currently, DNA Barcoding is being employed in large variety of organisms ranging from yeasts to human beings. Several workers have validated the effectiveness of DNA Barcoding. Barcoding promises to revolutionize our capacity to know an monitor biodiversity which has profound societal and economic impacts. It is immensely useful for identification of known species and discovery of new ones. DNA Barcoding approach ishelpfulinidentifyingaspecimenasbelongingtoa certain animal species based on the above single universal marker. Over the last 8 years, this approach has become increasingly popular and advances have been made in various groups of organisms. Establishing DNA Barcodes to identify previously defined groups of organisms at any taxonomic level is a powerful application of modern technology to biodiversity study. DNA Barcoding is a unique concept with many innovative attributes undertaking continuos improvement. It is not the goal, but the tool to be used in order to improve our understanding of the surrounding world. It is a fast reliable and cheap method for species identification and discovery. It provides permanent tags unchanged during taxonomic revisions. It will have multiple applications for marine life: Identificationoflarvae,invasivespecies, crypticspecies,new species, controlling illegal trade of protected species , stock management, biodiversity assessments, ecosystem monitoring, revisions of certain taxa, inference of phylogenetic relationships, phylogeographic and speciation patterns.